Alphinia Bio

Alphinia Bio

Alphinia Bio is a pre-clinical, deep tech, synthetic biology company.

We develop novel biological therapies and diagnostics.

Our proprietary platforms are specifically designed for bioengineering in mammalian cells.

We engineer unique systems using a partitioned RNA-virus to facilitate the directed evolution of biomolecules with new or enhanced functions.

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Directed evolution

is a powerful method used in molecular biology and bioengineering to mimic natural evolution’s process of mutation and selection to evolve biomolecules toward a user-defined goal.

It involves repeatedly subjecting a gene, protein, or organism to rounds of mutagenesis (to create genetic diversity) and selection (to enrich for variants with desired traits).

Directed evolution allows molecular function to be enhanced, or for the development of fundamentally new functions, such as increased catalytic activity, altered substrate specificity, improved stability, or novel binding properties.

Directed evolution can achieve outcomes not readily accessible through traditional bioengineering approaches.

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Each evolution campaign is broadly separated into seven stages of development:

  1. Design of evolutionary circuit.
  2. Synthesis of the gene or molecule as well as any corresponding targets; and their integration in Alphinia Bio’s platform.
  3. Testing of the synthesised circuit is then undertaken to ensure fidelity during an evolutionary campaign.
  4. Campaigns are then undertaken; whereby the validated circuit is exposed to the designed selection pressure.
  5. Genotyping is then undertaken to identify novel amino acid changing variants in the gene or biomolecule of interest
  6. Analysis of this genotyping data is undertaken to prioritise novel variants; which are then selected for synthesis or site-directed mutagenesis into the initial starting gene.
  7. Validation of new variants is then undertaken to prove an enhanced functionality. We ensure this validation occurs in a manner that recapitulates the selection circuit in which the biomolecule was evolved; as well as in the context with which the final molecule is envisaged to function.
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Licensing and partnerships

Please contact legal@alphiniabio.com to discuss partnering with Alphinia Bio, or to license our technology.

Order reagents

Reagents may be purchased directly from Alphinia Bio, for academic purposes and for industrial applications. Global shipping is available. Please note that ordering reagents is a multi-step process.

For academic customers

  1. Select required reagents.
  2. Enter your contact details.
  3. Enter your shipping details.
  4. Enter your project precis.
  5. We will contact you to confirm the details of your order, resolve any issues with your supporting documentation, and take payment.
  6. On receipt of payment and completed documentation, the selected reagents will be shipped to you.

For industry customers

  1. Select required reagents.
  2. Enter your contact details.
  3. We will contact you to schedule a meeting to discuss your requirements.

Available reagents

All plasmids are miniprep in 30µL volume (100–500 ng/µL) and are shipped at room temperature.

Reagent

CMV-tTA

Catalogue #

Ab-003

Description

Plasmid expressing tTA under a CMV promoter.

Reference

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Contacts

Business address:

→ 7B Wilks Rd, Lenah Valley TAS 7008, Australia
  • ACN 655 772 928
  • ABN 68 655 772 928
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